MSI LDPI Laser/Photochemical Ionisation Mass Spectrometry Imaging Source
Compatible with Agilent, AB SCIEX and Thermo mass spectrometers
- Compatible with mass spectrometers: Agilent, AB SCIEX and Thermo mass spectrometers
Spatial resolution: 2–3 μm
1. No polarity discrimination (suitable for the ionisation and imaging of non-polar substances);
2. High sensitivity;
3. No need for matrix spraying;
4. Operates at atmospheric pressure;
5. No matrix interference; capable of detecting compounds with a charge-to-mass ratio as low as 70 (m/z)
The MSI LDPI laser/photochemical ionisation mass spectrometry imaging source is an innovative mass spectrometry imaging system featuring patented technology; based on open-path laser desorption/ionisation (LDI)/post-photochemical ionisation (PCI) technology, the key to this technology lies in the combination of laser desorption/ionisation with a compact vacuum ultraviolet photochemical ionisation unit, enabling high spatial resolution imaging of a wide range of polar and non-polar molecules, and enhancing the detection of histamine, adenosine, choline, adenine, vitamin D3, cholesterol, glutamic acid, threonine, guanine, ceramides, and various lipids such as fatty acids, PE, PA, MAG, DG, HexCer and GalCer.
Under ambient pressure conditions, the system achieves ultra-high sensitivity; compared with laser ablation alone, signal enhancement can reach 1–3 orders of magnitude, whilst simultaneously achieving a breakthrough spatial resolution of 3 micrometres, giving it a strong competitive edge within the industry. The MSI LDPI-25 provides a molecular imaging solution at the single-cell scale for cutting-edge scientific fields such as life sciences, environmental toxicology and plant metabolism.
Application Areas
Medical Diagnostics: Mechanisms underlying the formation of melanocytic naevi; mechanisms of statin-induced cognitive impairment
Drug Development: Medicinal value of the traditional Chinese medicinal herb Atractylodes; pathogenic mechanisms in obese mice
Life Sciences: Imaging and analysis of endogenous metabolites; physiological roles of cholesterol in hippocampal brain tissue
Other: Industrial applications, forensic science, toxicology, environmental chemistry, archaeology, etc.
Performance Features
Product Features
Operates at atmospheric pressure; simple operating environment
Photochemical ionisation
Wider coverage of small-molecule samples (MW 70–1000)
High spatial resolution and high sensitivity
No polarity discrimination; comprehensive coverage of both polar and non-polar molecules in the sample
Detects a wider range of analytes than conventional MALDI and AP-MALDI
No matrix required, thereby avoiding background interference caused by matrix molecules
Performance Specifications
Spatial resolution: 2–3 μm
Laser wavelength: 355 nm
Laser frequency: 1000 Hz
Ionisation source: Kr lamp
Compatible brands
Application Examples
Melanoma Imaging
Results showing the distribution of metabolites in the stratum corneum, stratum granulosum, tumour and normal tissue of melanoma skin samples. Analysis of melanoma tissue sections revealed metabolic heterogeneity in the melanoma microenvironment and demonstrated the phenomenon of abnormal proliferation and invasion by tumour cells.
Mouse Hippocampal Tissue Imaging
It is capable of distinguishing between different regions of the mouse hippocampus and the spatial distribution of various metabolic molecules; following optimisation, metabolites in mouse hippocampal tissue can be imaged with a spatial resolution of 4 μm.
Anal. Chem. 2024, 96, 14
Fossil mass spectrometry imaging
Lacquerware mass spectrometry imaging
Mass Spectrometry Imaging of Mouse Retinas
New Ceyuan Instruments | Your ideal partner for mass spectrometry! Making molecular spatial imaging simpler and scientific research testing more efficient!